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Hyaluronic acid is applied in food field, began in Japan and the United States market, in 1996, Japan's health ministry announced the existing additive list, it contains a comb extraction and microbial fermentation of hyaluronic acid from two sources, around 2000, the Japanese with hyaluronic acid to begin large-scale food market, the products are mainly aimed at improving the skin moisture, beauty to raise colour, in addition to health food, the Japanese market also appeared a lot of type of ordinary food contains hyaluronic acid, such as fruit juice, soy milk, etc.; Subsequently, the United States began to appear on the market with hyaluronic acid of health care products, and as the third generation after ammonia sugar, chondroitin products to improve the function of knee joint, get the wide recognition of consumers.
Test items
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Specifications
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Appearance
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white powder
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Solubility
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Soluble in water, insoluble in acetone and ethanol
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Glucuronic acid
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≥44%
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Sodium hyaluronate
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≥91%
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Light transmittance (0.1% Aqw. solution)
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A600nm≤ 0.01
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Nucleic acid (0.1% Aqw. solution)
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A260nm≤ 0.5
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pH (0.1% Aqw. solution)
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6.0-7.5
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Molecular weight
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(1.0-1.5 )* 106 Da.
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Protein
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≤0.1%
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Loss on drying
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≤10%
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Residue on Ignition
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≤20%
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Cl
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≤0.5%
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Fe
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≤80ppm
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Heavy metal
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≤20ppm
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As
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≤2ppm
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N
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3.0-4.0%
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Total number of bacteria
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≤10CFU/g
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Mold and yeast
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≤10CFU/g
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Staphylococcus aureus
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negative
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Pseudomonas aeruginosa
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negative
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Hemolysis
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negative
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Hemolytic streptococcus
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negative
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Product features:
Name of the original bacteria: Streptococcus zooepidemicus
Molecular weight (Dalton):
1, Low molecule: 2000 - 100,000 Dalton
2, medium Molecular weight: 0.8 - 1.6* 106 Dalton
3, macromolecule: > 2.0×106 Dalton
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